Larger Image Fig. 4. Interference with the PCP pathway prevents Shroom-induced apical constriction. Early embryos were animally injected with FLAG-Shroom RNA (50 pg) and other RNAs or MOs as indicated. (A-D) Vangl2 depletion inhibits Shroom-dependent ectopic apical constriction (arrowhead). CO, control. (A-C) Representative embryo images at stage (st) 10.5. (D) Quantification of the data, numbers of embryos with strong, mild and no ectopic constriction are presented. Strong (++) apical constriction involves hyperpigmentation and the accompanying tissue invagination, mild (+) constriction is frequently visible as diffuse hyperpigmentation in the absence of tissue indentation. (E-H) Inhibitory effects of N-Daam1 and zPk1δPL on Shroom-dependent apical constriction in stage 10 embryos. (H) Quantification of the data. +indicates ectopic apical constriction; ‘normal’ indicates no constriction. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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