Fig 7. Global reduction of VICKZ in Xenopus embryos inhibits CNC migration non-cell autonomously. (A) A scheme illustrating the position of blastomere a2 in a 32-cell stage embryo that gives rise to most of the CNC cells in the embryo. (B,C) 32 cell-stage embryos were injected in a2 with Alexa-red and either control morpholino (CMO) (B) or xVICKZ antisense morpholino (AMO) (C) and allowed to develop to tailbud stage. Both AMO-and CMO-injected cells reach their homing sites in the branchial, hyoid, and mandibular arches (b.a., h, m). (D-F) CNC orthotopic transplantations. Wild–type CNC grafts (in green) exhibited normal migration in control embryos (D), with grafts taken from a rostral position in the neural folds migrating along the mandibular migration pathway (D1) whereas grafts from more caudal position migrating to the branchial arches (D2). Grafts taken from xVICKZ-depleted embryos in orange, were able to migrate properly in WT embryos (E). However, wild-type CNC (green) were unable to migrate in xVICKZ AMO-injected embryos (orange). Abbreviations: m, mandibular; h, hyoid; b.a, branchial arches. doi:10.1371/journal.pone.0136408.g007
Image published in: Carmel MS et al. (2015)
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