FIG. 2. Semitic tissue in Xenopus dorsoanterior phenotypes. Dot blots were used to determine the reactivity of embryos (a, b) or adult tissues (c, d) to 12/101, an antibody which recognizes Xenows myotubes. (a) Normal (DA1 5) embryos have the highest reactivity while dorsoanterior deficient (DA1 0,3) and enhanced (DA1 8,lO) embryos have less reactivity. (b) Control blots-identical samples were dotted as in (a), but were processed without primary antibody. (c) The first four squares are Xenopus adult skeletal muscle samples diluted to 10 mg/ml wet weight (M), 5 mg/ml (M/2), 1 mg/ml (M/10), 0.01 mg/ml (M/1000). The other two squares are Xenopus liver samples diluted to 10 mg/ml wet weight (L) and 5 mg/ml (L/2). (d) Samples processed as in (c), but without primary antibody.
Image published in: Kao KR and Elinson RP (1988)
Copyright © 1988. Image reproduced with permission of the Publisher, Elsevier B. V.
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