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Supplemental Figure S8 Co-immunoprecipitation of proteins overexpressed in HEK293T cells showing that mutation of the RING finger of HA-tagged Ectodermin does not impair Flag-Smad4 interaction. Anti-HA western blotting of anti-HA pull-downs show comparable immunoprecipitation of Ecto wild-type and EctoCAmut proteins. Smad4 (anti-Flag WB) interacts similarly with both.

Image published in: Dupont S et al. (2005)

Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V.

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