Fig. 2. In situ hybridization for epidermal markers in exosc9 and ptbp1 morphants. Albino embryos were injected with the control morpholino (MoCo, A, D, G, J), MoPtbp1 (B, E, H, K) or MoExosc9 (C, F, I, L) in both blastomeres. Embryos were fixed at stage 32 and processed for in situ hybridization with the following probes: tuba1a (A–C), foxa1 (D–F), foxi1 (G–I) and itln2 (J–L).
Image published in: Noiret M et al. (2016)
Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| tuba1cl.3 | alphatub84, b-alpha-1, lis3, tuba1a, tuba1a-a, tuba1a-b, tuba3 | X. tropicalis | Throughout NF stage 32 | epidermis ciliated epidermal cell |
| foxa1.L | fkh2, foxa1-a, foxa1-b, foxa1b, HNF-3 alpha, HNF3 alpha, HNF3-alpha, hnf3a, HNF3alpha, tcf3a, tFoxA1, xfkh2 | X. laevis | Throughout NF stage 32 | epidermis notochord tail tip chordoneural hinge foregut hindgut mesoderm endoderm secretory epithelial cell |
| foxi1.L | ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 | X. laevis | Throughout NF stage 32 | epidermis cloaca ionocyte |
| itln2.L | hl-2, hl2, intel 2, intel2, intelectin-2, Xeel | X. laevis | Throughout NF stage 32 | epidermis goblet cell dorsal fin tail fin |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + ptbp1 MO + NF32 (morphology) | fig.2.b, e, h, k |
| ||
| Xla Wt + exosc9 MO + NF26 (morphology) | fig.2.c, f, i, l |
| ||
| Xla Wt + exosc9 MO + NF32 (morphology) | fig.2.c, f, i, l |
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