Figure 2. and Movies 1 and 2 Long-range intercellular calcium waves in the dorsal marginal zone. (a) Individual frames from confocal time-lapse of the large propagating calcium wave in Movie 1 (see supplementary material). Time points are indicated in white. Cell mixing produces a mosaic pattern of labeled (bright) and unlabeled (black) cells. A large calcium wave initiates (t = :00), travels ∼20 cell diameters (t = 1:00), and subsides over approximately eight minutes (t = 8:00). The scale bar indicates 50 μm in this and all subsequent figures. (b) The plot of δF/F0 for each of the areas shown in the colored boxes in the last panel of (a). The plot shows the propagatory nature of the rise in calcium levels and the more even recovery. (Movie 1) This movie shows the patterns of calcium release in a DMZ explant labeled on the left side with calcium green dextran; the right side is unlabeled and is black, though cells are present in the field of view. In this and all DMZ movies, the dorsal lip of the blastopore is at the bottom of the screen, and the dorsal midline runs vertically through the middle of the screen. The mediolateral axis is horizontal. As the movie begins, several small flashes of calcium release can be observed throughout the explant (described in Figure 4b). About halfway through the movie, a small intercellular calcium wave arises near the midline of the explant, followed closely by another, much larger calcium wave. Still frames depicting the large wave in this movie are presented in Figure 1a. (Movie 2) This movie shows a DMZ explant in which all the cells are labeled with calcium green dextran. This explant undergoes a small wave, then a larger wave, then another small wave. These waves are less dramatic than those in Movie 1, possibly due to less effective calcium green dextran loading
Image published in: Wallingford JB et al. (2001)
Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V.
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