Data generation, mRNA content of the embryo and gene expression dynamics, see also Fig S1, S2 A – Experimental design and sample collection B – Left: eef1a1o and ERCC-00171 abundance in relative normalization (TPM), Clutch A polyA+. RNA standard decreases with time. Right: absolute normalization of left. Red line indicates ERCC-00171 transcripts added based on the manufacturer’s datasheet. The RNA standard is constant at the correct abundance. C – Total mRNA (PolyA+ and rdRNA) in the embryo in nanograms with time. Gray region and line respectively mark Gaussian process 95% confidence interval (CI) and median of Clutch A & B PolyA+ data. D – Dynamics of gene expression of PolyA+ RNA in transcripts per embryo. Circles mark Clutch A PolyA+, triangles mark Clutch B PolyA+. The horizontal axis marks time (bottom) and NF developmental stage (top). Shaded regions mark Gaussian process 95% CIs of the data.
Image published in: Owens ND et al. (2016)
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