Figure S1. Analysis of the expression pattern of Noggin4 and its ability to induce secondary axes. (a-f) The expression pattern of Noggin4 on successive stages, from the late blastula (stage 9) till early-midneurulas (stage 14), as revealed by the whole-mount in situ hybridisation. Note, that at all these stages, a maximum of the expression is located within the presumptive anterior neural plate. All embryos are shown from the vegetal pole, anterior to the top. (c’–f’) Vibratom sections of embryos at stage 10.5, 11.5 and 14 made as indicated on (c and e) by dashed lines. (g) Control in situ hybridization with sense probe to Noggin4 mRNA. (h and h’) Injection of 15 ng/blastomere of Noggin1 mRNA mixed with FLD, into the equatorial zone of ventral blastomeres at 4-cell stage induces formation of secondary body axes (98%, n=120; red asterisk— main axis; yellow asterisk— secondary axis). (i and i’) By contrast, injections by the same way of even 150 ng/blastomere of Noggin4 mRNA did not induce secondary axes at all (0%, n=120; asterisk— main axis).Abbreviations: bl — blastopore, dbl — dorsal blastopore lip, n —notochord, np— neural plate, s — somite. Bar everywhere is 100 m.
Image published in: Eroshkin FM et al. (2016)
Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nog4.S | nog-4, noggin 4 | X. laevis | Throughout NF stage 10.25 | upper blastopore lip |
| nog4.S | nog-4, noggin 4 | X. laevis | Throughout NF stage 10.5 | upper blastopore lip |
| nog4.S | nog-4, noggin 4 | X. laevis | Throughout NF stage 11.5 | neural plate upper blastopore lip |
| nog4.S | nog-4, noggin 4 | X. laevis | Throughout NF stage 12.5 | neural plate neuroectoderm neuroectoderm outer layer |
| nog4.S | nog-4, noggin 4 | X. laevis | Throughout NF stage 14 | pre-chordal neural plate |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + nog + NF14 (morphology) | fig.S1.h |
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