Fig. 7. BMP inhibition promotes the MCC fate in regenerating HAECs. (A-C,J,K) Acetylated tubulin-positive, morphologically normal MCCs increased following BMP pathway inhibition by treatment with Noggin (100 ng/ml) in HAEC cultures from healthy donors (A-C) or cystic fibrosis patients (J,K). (D,E) SEM images of control or Noggin-treated HAEC cultures at stage LC. (F) The number of MCCs per field of observation in HAEC cultures from three donors at various time points of culture. Noggin and dorsomorphin (Dorso.) induced precocious and increased rates of ciliogenesis. CTR, control (G) FACS confirmed the increase in MCCs and goblet cells in Noggin-treated normal HAEC cultures. (H) The maximal increase in the number of MCCs relative to untreated cultures was observed when Noggin treatment of HAECs started at Po stage (ALI days 6-9). (I) Noggin treatment increases the number of MCCs in HAEC cultures from cystic fibrosis (CF) donors. (G-I) Data are mean±s.d. from three independent experiments; *P<0.05; **P<0.01; ***P<0.001; ns, not significant; Student’s t-test.
Image published in: Cibois M et al. (2015)
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