Fig. S2. Inhibition of blastopore lip formation in Vangl2-depleted embryos. (A-E) Early embryos were injected with Vangl2 MO or control MO either dorsally or ventrally as indicated. LacZ RNA (150 pg) has been coinjected a lineage tracer. Vegetal views of stage 11 embryos are shown. E, Quantitation of the experiments shown in A-D. (F, G) En face view of the dorsal lip in stage 10+ embryos. Vangl2 was depleted by a unilateral injection of control or Vangl2 MO (30 ng each). F-actin staining in control (F) and Vangl2-depleted (G) blastopore cells. Arrow points to reduced apical F-actin in Vangl2-depleted cells (marked by GFP, green). Animal pole is at the top. Scale bar in F (also applies to G) is 10 μm. Dashed line indicates the midline. Arrowheads point to F-actin-enriched areas, which are reduced by Vangl2 depletion.
Image published in: Ossipova O et al. (2015)
Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + vangl2 MO + NF11 (morphology) | fig.s2.b |
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| Xla Wt + vangl2 MO + NF11 (morphology) | fig.s2.d |
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| Xla Wt + vangl2 MO + NF11 (immunohistochemistry) | fig.s2.g |
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