Figure 2. Characterization of the SUR1(QIL/VFY) mutant. (A) Diazoxide dose–response relationships for Kir6.2/SUR1 (circles) and Kir6.2/SUR1(QIL/VFY) (triangles). Currents were normalized to the current measured in 0 ATP. Hill equation fitting yielded K1/2 = 132 μmol/L (h = 1.65) for Kir6.2/SUR1. (B) Response to 100 μmol/L MgADP of SUR1 wt and SUR1(QIL/VFY). Currents were normalized to the current measured in the absence of nucleotides immediately before MgADP application. Numbers indicate the number of patches included in each average. (C) Representative patch-clamp recordings illustrating the responses of wild type and SUR1(QIL/VFY) channels to 100 μmol/L MgADP in the absence of ATP. (D) Current amplitudes in the absence of nucleotides of Kir6.2/SUR1, Kir6.2/SUR1(QIL/VFY), Kir6.2/SUR2A, and Kir6.2/SUR2A(EIL/VFY). (E) ATP dose–response relationships for Kir6.2/SUR1 (circles) and Kir6.2/SUR1(QIL/VFY) (triangles). Currents were normalized to the current measured in 0 ATP. Hill equation fitting yielded K1/2 = 20 μmol/L (h = 1.33) for Kir6.2/SUR1 and K1/2 = 17 μmol/L (h = 1.32) for Kir6.2/SUR1(QIL/VFY). SUR1, sulfonylurea receptor 1.
Image published in: Principalli MA et al. (2015)
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