Fig. 8. Axonal Tctp interacts with pro-survival Mcl1. (A) Coronal section of stage 43 retina probed with an anti-Mcl1 antibody and counterstained with DAPI. (B,C) PLA signal for Tctp and Mcl1 in cultured rat cortical neurons (E18.5+3 DIV) counterstained with DAPI and phalloidin. The boxed areas are enlarged beneath. In C, anti-Mcl1 serum and blocking peptide were co-incubated before proceeding with the assay. (D) Representative control and Tctp morphant RGC growth cones stained for P53. Mean±s.e.m.; n, number of growth cones analysed; ***P=0.0002, unpaired t-test. (E) Representative control and Tctp morphant RGC growth cones stained with an antibody that specifically recognises the cleaved (activated) form of Caspase-3. Mean±s.e.m.; n, number of growth cones analysed; ***P=0.0002, unpaired t-test. Scale bars: 50 μm in A; 10 μm in B,C; 5 μm in D,E.
Image published in: Roque CG et al. (2016)
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