Figure 2. Amplicon sequencing analysis of on-target sites in founder animals. (A) Representative Integrative Genomics Viewer (IGV) images at each target site. Deletions, insertions and base substitutions are indicated by black, purple and other four color (red, blue, green and ocher) bars, respectively. (B) Positions of mutations at the tyr, slc45a2 and ltk target sites. The horizontal and vertical axes indicate the relative positions from the predicted cut site, 3 bp upstream of protospacer-adjacent motifs (PAMs) and the occupancy rate of mutant alleles to the total reads at each base position, respectively. The number of mutations was counted by IGV tools.
Image published in: Shigeta M et al. (2016)
Copyright © 2016. Image reproduced with permission of the Publisher, John Wiley & Sons.
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