Figure 6. Detection of off-target activity by heteroduplex mobility assay (HMA). (Left) Putative off-target sequences of tyr, slc45a2 and ltk are aligned below the on-targets. The protospacer-adjacent motif (PAM) sequences and mismatch bases are shown as red and blue-small characters, respectively. (Right) Gel images converted from electropherograms of five off-target sites of each gene analyzed by HMA. On and OT mean on-target and off-target sites, respectively. Note that sgRNA targeting slc45a2 has two mismatches at the 5′ of the protospacer sequence (underscored). An asterisk in slc45a2 OT2 indicates a faint band. Abbreviations; Wt, wild type; S, severe phenotype.
Image published in: Shigeta M et al. (2016)
Copyright © 2016. Image reproduced with permission of the Publisher, John Wiley & Sons.
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