Figure 5. Correlation between somatic mutation rates determined by RFLP and amplicon sequencing analyses. The results of RE or RGEN-RFLP analysis were plotted against those of amplicon sequencing from tyr (N = 37)- and slc45a2 (N = 30)-disrupted animals with various phenotypes. In the case of slc45a2, both RGEN-RFLP and RE-digested RFLP were analyzed in parallel. Somatic mutation rates estimated by RFLP analyses exhibited a strong correlation with those determined by amplicon sequencing. In the case of ltk, although we could not statistically evaluate the association because of biased sampling (half and full phenotypes, N = 14), the results also indicate a positive association. R2 is the square of the Pearson correlation coefficient.
Image published in: Shigeta M et al. (2016)
Copyright © 2016. Image reproduced with permission of the Publisher, John Wiley & Sons.
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