Supplemental Figure S2. (A) Transcript levels of gcn5 and gapdh transcript levels in TBP, TLF, TBP2 triple knockdown (TKD) embryos and control embryos, as determined by RT‐qPCR. (B) Reactivity of C26A10 antibody with Xenopus Gcn5 in embryos injected with 1 ng of gcn5 mRNA, Gcn5‐AS oligonucleotide and in control embryos (weak signal). No cross‐reactive bands are observed. TBP is shown as control. For validation of TBP, TBP2 and RNAPII antibodies see (Akhtar and Veenstra, 2009; Jallow et al., 2004; Veenstra et al., 1999). (C) TUNEL assay for the detection of apoptosis in control and Gcn5‐AS‐injected embryos. (D) Morphology of non‐injected and water‐injected controls, Gcn5‐AS‐injected and rescue (Gcn5‐AS rescued with co‐injected full length human GCN5 mRNA or GCN5‐ΔHAT mRNA encoding a truncated GCN5 protein lacking the histone acetyltransferase domain) embryos are shown. Photos were taken at stages 11.5 and 23 (controls). (E) Summary of survival (normal development) statistics after embryo collection at stage 10.5 and 23. Gcn5-AS embryos were rescued by full length human GCN5 mRNA at a rate of 80-86% at stage 10.5 and 38-72% at stage 23. Embryos rescued with GCN5-ΔHAT exhibited 64-91% rescue efficiency at stage 10.5 and 21-30% at stage 23. (F) Gene ontology analysis of decreased transcripts in Gcn5-AS embryos.
Image published in: Gazdag E et al. (2016)
Image downloaded from an Open Access article in PubMed Central. © 2016. Published by The Company of Biologists Ltd
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