Movie 2. Calcium imaging inert hearts produced by adprhl1 morpholino. Time-lapse movie of a stage 42 tadpole that was co-injected with Adprhl1-e2i2MO and also R-GECO1 RNA into D-2/4 blastomeres. The injection masses were 20 ng morpholino and 800 pg RNA. Ventral view of the cardiac ventricle is displayed, anterior to left. The small tubular heart of this tadpole is mechanically inert. Nonetheless, the red fluorescent calcium indicator protein, R-GECO1, detects an active intracellular calcium wave. The wave initiates at the inflow region, which is positioned more dorsally than the ventricle so appears fainter (immediately to the right of the ventricle in the movie). It then propagates through the ventricular portion of the looped tube (from top to bottom of movie) and outflow tract (left) without apparent obstruction. Greyscale images were captured and false-coloured in Image J using the GEM LUT. Movies are 10 s duration, captured at 20 frames per second and are played back at 5 fps.
Image published in: Smith SJ et al. (2016)
© 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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