Fig. 3 – Inducible expression of GFP-APP in transgenic Xenopus. Fluorescent images of tadpoles microinjected in the first-cell stage with the two constructs depicted on the left of the images. Gene expression was induced by incubating the animals in the presence of doxycyclin for 12 h. No fluorescence was observed after doxycyclin treatment using the construct encoding GFP-APP (upper right panel). Using the construct encoding GFP alone, fluorescence was observed in neuronal cells of the transgenic tadpoles after doxycyclin treatment (lower right panel). The transmembrane domain of APP is indicated by a dashed box. Tet: Tet activator protein; pTRE: promoter containing Tet-responsive elements. SP: signal peptide sequence; pA: poly-adenylation signal.
Image published in: Collin RW and Martens GJ (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
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