Fig. S1. Notch-expressing cells do not contribute to notochord. NICD-myc mRNA was injected with (B, C) or without (E, F) β-galactosidase mRNA as a lineage tracer into both dorsal blastomeres to target the midline. Embryos were fixed, bisected transversely, stained for lacZ (pink, A–C), and processed by ISH for AxPC expression in the notochord (purple, A–F). This injection technique efficiently targets the midline (A), with labeled cells in FP, notochord, dorsal endoderm and sometimes medial somite. Embryos with activated Notch have reduced (B, E) or absent (C, F) notochord. Co-injected lineage tracer never labels any notochord that forms (B). Staining for NICD protein (αnti-myc, brown in E, F) shows that NICD+ cells never give rise to the notochord.
Image published in: Peyrot SM et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| pcdh1.L | axial protocadherin, axpc | X. laevis | Throughout NF stage 13 to NF stage 21 | notochord neural plate |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||||
|---|---|---|---|---|---|---|
| Xla Wt + canotch1 + NF13-21 (in situ hybridization) | fig.S1.b, c, e, f |
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| Xla Wt + canotch1 + NF13-21 (in situ hybridization) | fig.S1.b, e |
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| Xla Wt + canotch1 + NF13-21 (in situ hybridization) | fig.S1.c, f |
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