Larger Image Fig. 2. Characteristic features of no privacy mutant. (AF, E) The same two sibling F2 embryos derived from an intercross of heterozygous F1 mutant carrier frogs (stock 534) were observed from stage 39/40 to stage 50 as indicated in the panels. (AE) Side views of the mutant (top) and wildtype (bottom) during development. (E) Ventrolateral view of the same embryos shown in (E), illustrating that the mutant (right) has a see-through belly due to lack of pigmented iridophores. Dorsal view (F, left: wildtype; right: mutant) and ventrolateral view (F, mutant only) of the same embryos as seen in (AE, E) at stage 50. Dorsal (G) and lateral (G) views of another mutant embryo and lateral view (H) of another wildtype embryo at stage 53, both of which are siblings of those above, where in white-dotted circles, the forelimbs, and in white-dotted ovals, the hindlimbs, are marked. The panel G was composed of two different focal planes of the same embryo. Image published in: Nakayama T et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xtr Wt + ENU + NF48-49 (whole-mount microscopy) Fig 2. E E' Anatomical Phenotype abnormal ventral posterior hindgut endoderm absent iridophore decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF39-40 (whole-mount microscopy) Fig. 2 A Anatomical Phenotype decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF42 (whole-mount microscopy) Fig. 2 B Anatomical Phenotype decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism decreased size of the eye Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF44-45 (whole-mount microscopy) Fig. 2 C Anatomical Phenotype decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF46-47 (whole-mount microscopy) Fig. 2 D Anatomical Phenotype decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism decreased size of the eye Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF50 (whole-mount microscopy) Fig. 2 F F' Anatomical Phenotype absent iridophore decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF53-54 (whole-mount microscopy) Fig. 2 G Anatomical Phenotype absent iridophore decreased pigmentation in the epidermis decreased pigmentation in the whole organism Disease Hermansky-Pudlak syndrome Xtr Wt + ENU + NF53-54 (whole-mount microscopy) Fig. 2. G' H Anatomical Phenotype absent iridophore decreased pigmentation in the epidermis decreased pigmentation in the eye decreased pigmentation in the whole organism Disease Hermansky-Pudlak syndrome Permanent Image Page Printer Friendly View XB-IMG-154024