Fig. 3. Gastrula dissection strategy and validation by marker gene expression in RNA-seq data. A. Illustration shows the locations of eyebrow hair knife cuts (dotted lines) for early gastrula dissections. Left shows a sagittal view while right shows a vegetal view. Dorsal is to the right. B. A heat map from RNA-seq data shows the relative expression of various marker genes in the different dissected embryo fragments. Abbr. AC, animal cap; DMZ, dorsal marginal zone; LMZ, lateral marginal zone; VMZ, ventral marginal zone; VEG, vegetal mass.
Image published in: Blitz IL et al. (2017)
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