Figure 3 Temporal requirement of Notch/Delta signaling during early neural crest development. A–G: Groups of NC explants were isolated from hairy constructs mRNA injected embryos and cultured from stage 12.5 to 14. Inset in A–C and E–G: the fluorescent picture verifies the injection. A–C: hairy-GR activator constructs inject explants strongly express pax3. D–G: DNhairy-GR constructs inject explants showed no changes with respect to control NC (D). H–O: Neural crest explants (NC) were prepared by dissection of the neural crest. Groups of explants were fixed immediately after excision (left), at stages 13 or 17. Groups of explants were cultured until stages 17 or 22 in the presence or absence of DAPT (see text for details). H,L: NC explants removed from embryos at different stages express foxd3 when fixed at the moment of dissection. I: NC explants dissected from stage 13 embryos and cultured until stage 17 express foxd3. J: The NC explants isolated at stage 13 and cultured until stage 17 in the presence of 100 μM DAPT lose foxd3 expression. K: The hairy2b-GR mRNA strongly rescued foxd3 expression of DAPT treated NC explants. M: NC explants dissected at stage 17 cultured until stage 23 shown foxd3 expression. N: DAPT treatment of NC explants isolated from stage 17 embryos produced inhibition of foxd3 expression. O: The hairy2b-GR mRNA strongly rescued foxd3 expression of DAPT treated NC explants.
Image published in: Vega-López GA et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
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