Figure 7 hairy genes do not control cell proliferation during neural crest specification. A–R: Dorsal views of Xenopus laevis embryos; anterior side is on the right. The injected side is indicated by an arrowhead. Embryos injected into one blastomere at the 8- to 16-cell stage with 700 ng of hairy constructs mRNA were treated with dexamethasone and HUA (hydroxiurea 20 mM, aphidicolin 150 μM) at stage 11 until neurula stages and fixed. A–L: hairy inducible constructs mRNA or DNhairy constructs mRNA-injected embryos were incubated in the presence of HUA inhibitors mix until the neurula stage. The expression of foxd3 was analyzed by in situ hybridization. hairy inducible constructs produced an expanded foxd3 labeling (A–C) and DNhairy constructs caused a decrease in foxd3 labeling (D–F). hairy constructs reduced the expression of sox2 marker in the neural plate (G–I) and DNhairy constructs produced an expansion in sox2 labeling (J–L). The presence of HUA caused no changes in the foxd3 and sox2 phenotype after hairy gain or loss of function. M–R: Whole-mount anti-phospho H3 immunohistochemistry labeling was performed as indicated in the Experimental Procedures section. The injected side can be recognized by the fluorescence of the lineage tracer fluorescein dextran and is indicated by a white arrowhead. S: Quantification of phospho-H3 labeling for hairy constructs-microinjected embryos. No significant changes were observed in cell proliferation caused by the microinjection of hairy or DNhairy inducible constructs. T: No significant anti-histone H3 staining was observed in embryos treated only with HUA.
Image published in: Vega-López GA et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| foxd3.L | fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 | X. laevis | Sometime during NF stage 15 to NF stage 16 | neural crest cranial neural crest pre-chordal neural plate border neural plate border |
| sox2.L | anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 | X. laevis | Sometime during NF stage 15 to NF stage 16 | neural plate |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + hes1-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.a |
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| Xla Wt + hes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.b |
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| Xla Wt + hes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.c |
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| Xla Wt + dnhes1-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.d |
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| Xla Wt + dnhes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.e |
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| Xla Wt + dnhes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.f |
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| Xla Wt + hes1-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.g |
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| Xla Wt + hes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.h |
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| Xla Wt + hes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.i |
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| Xla Wt + dnhes1-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.j |
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| Xla Wt + dnhes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.k |
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| Xla Wt + dnhes4-GR + DEX + aphidicolin + hydroxyurea + NF16 (in situ hybridization) | fig.7.l |
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