Fig. 2. Time course of treatment with factors. (A) Ectoderm taken from late blastula (stage 9) embryos was either treated immediately or aged to early (stage 10.25), mid- (stage 11) or late (stage 12) gastrula stage and treated with 50 ng/ml bFGF (F), 1 mg/ml noggin (N), the combination (N+F), activin (A) or with an LCMR control (L). Explants were subsequently cultured to stage 22, and harvested for RT-PCR analysis. Expression of NCAM, En-2, Krox 20, Hox B9, Muscle actin and Collagen Type II was analyzed. (B) Ectoderm was treated identically as in A except, stage 12 ectoderm was not treated since the explants were harvested at this stage to analyze expression of the early mesoderm markers, Xbra and Goosecoid. EF-1a expression is shown as a loading control in both panels.
Image published in: Lamb TM and Harland RM (1995)
Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Permanent Image Page
Printer Friendly View
XB-IMG-154778