Fig. 3. Embryos from frozen sperm develop normally. A) Eggs were fertilised using sperm frozen by the methods shown and stored on LN2 for 3 months, the number of normal and abnormal embryos was scored at NF32, normal embryo percentages are expressed as a % of the surviving embryos at NF32. Means ± SEM are shown. B) Example of normal, left, and abnormal, right, embryos fertilised with cryopreserved sperm. C) Embryos produced using fresh or frozen sperm (Harland method) were fixed at NF32 and 12 of each analysed for the expression of the markers shown by in situ hybridisation. D) Once the embryos had developed to metamorphosis they were weighed. Means ± SEM are shown.
Image published in: Pearl E et al. (2017)
© 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hhex.L | hex, tHex, XHex | X. laevis | Throughout NF stage 32 | foregut |
| gja3.L | alpha3 connexin, connexin 46, connexin46, Cx40.4, cx46 | X. laevis | Throughout NF stage 32 | somite epaxial muscle |
| lmo2.L | LMO-2, xlmo-2, xlmo2 | X. laevis | Throughout NF stage 32 | ventral blood island tail bud blood vessel |
| tbx20.L | XTBX20 | X. laevis | Throughout NF stage 32 | heart |
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