Fig. 1. Both CENP-C and CENP-T promote Sgo1 recruitment to centromeres. a Representative examples of replicated chromosomes assembled in the egg extracts and stained with the indicated antibodies. Insets highlight an individual centromere within each chromosome mass. Scale bar, 10 μm. b Immunoblot analysis of the extracts used to assemble the chromosomes shown in (c–e). Increasing amounts of mock-depleted CSF extract, expressed as percentage, and aliquots of extracts depleted with specific antibodies, as indicated, were analyzed side by side to estimate the extent of each depletion. H1 served as a loading control. c–e Representative examples of chromosomes assembled in the indicated extracts and stained with antibodies against Sgo1 (c), Bub1 (d), and phosphoH2A (pH2A) (e). For validation of the pH2A antibody see Online Resource 1. Scale bar, 10 μm. f Quantification of average fluorescence in centromere pairs per nucleus (chromosome mass), expressed as a percentage of the average obtained in mock depleted extracts. Bars represent mean ± SD. More than ten nuclei were measured per condition in each of the three independent experiments
Image published in: Williams SJ et al. (2017)
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