S2 Fig. Whole Mount In Situ Hybridization (WISH) for different Hox genes after Hox ectopic expression. (A) The expression of HoxD-1, HoxB-4, HoxC-6, HoxA-7 and HoxB-9 is shown for WT (a-e), HoxD-1 injected (f-j) and HoxA-7 injected (k-o) embryos. White arrows point to the anterior borders of gene expression. In HoxD-1 injected embryos, all the genes examined were anteriorised (f: n = 8/12; g: n = 10/13; h: n = 9/16; i: n = 9/13; j: n = 10/15). In HoxA-7 injected embryos, HoxD-1 (k, n = 5/10), HoxB-4 (l, n = 6/14) were repressed, HoxC-6 (m, n = 15/15) was not affected, and HoxA-7 (n, n = 11/14) and HoxB-9 (o, n = 14/16) were anteriorised. (B) The expression of HoxD-1, HoxD-3, HoxB-4, HoxC-6, HoxA-7 and HoxB-9 is shown for WT (a-f) and HoxB-4 GR (activated at st.8) injected (a’-f’) embryos. White arrows point to the anterior borders of gene expression. In HoxB-4 injected embryos, the expression of HoxD-1 (a’, n = 9/17) and HoxD-3 (b’, n = 9/14) were repressed, whereas the expression of HoxB-4 (c', n = 9/15), HoxC-6 (d', n = 10/18), HoxA-7 (e', n = 8/13) and HoxB-9 (f', n = 18/25) were anteriorised. (C) The expression of HoxD-1, HoxB-4, HoxC-6, HoxA-7, HoxB-9 and HoxC-12 is shown for WT (g-l) and HoxB-9 injected (g’-l’) embryos. In HoxB-9 injected embryos, the expression of HoxD-1 (g’, n = 4/9), HoxB-4 (h’, n = 11/12), HoxC-6 (I’, n = 14/14) and HoxA-7 (j’, n = 6/14) were repressed, whereas the expression of HoxB-9 (k’, n = 12/12) and HoxC-12 (I’, n = 9/10) were anteriorised. https://doi.org/10.1371/journal.pone.0175287.s003
Image published in: Zhu K et al. (2017)
© 2017 Zhu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hoxd1.L | MGC131028, Xhox.lab, xhox.lab1, xhoxd1, xlab, Xlab-1 | X. laevis | Throughout NF stage 28 | spinal cord |
| hoxd3.L | hox-d3 | X. laevis | Throughout NF stage 28 | spinal cord tail bud |
| hoxb4.L | LOC494794, XHox-1a, XHox-b4, xHox1a | X. laevis | Throughout NF stage 28 | spinal cord |
| hoxc6.L | ACI, xeb1, XlHbox1 | X. laevis | Throughout NF stage 28 | spinal cord somite trunk epaxial muscle tail bud |
| hoxa7.L | antp, Hox-A7, hox36, Xhox-36, xlhbox 3, XlHbox-3, XlHbox5 | X. laevis | Throughout NF stage 28 | spinal cord |
| hoxb9.L | hox 2.5, xhoxb9, XlHbox-6, XlHbox6 | X. laevis | Throughout NF stage 28 | spinal cord tail bud |
| hoxc12.L | xhoxc12 | X. laevis | Throughout NF stage 28 | posterior |
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