Supplementary Fig. S1. (A) Domain structures of two splicing variants of xDel1. The shorter variant (xDel1-E2) is generated by alternative splicing (in-frame) within the first EGF repeat. SP, signal peptide; RGD, the integrin-binding RGD motif. (B) Quantitative PCR analysis of expression level of xDel1 mRNA in DMZ and VMZ tissues. There is no substantial difference between them. Chd, positive control as a dorsal gene; Szl, positive control as a ventral gene. Although xDel1 expression appeared slightly stronger on the dorsal side in whole-mount in situ hybridization ( Fig. 1C), it is likely that it reflected the higher cell density on the dorsal side.
Image published in: Arakawa A et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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