Supplementary material Supplemental Fig. 2: Wbp2nl MO sequences and their alignment to mRNAs. Top: Sequence of the endogenous wbp2nl mRNA including 5′UTR (green) and ORF (black). Translational start site is underlined. Middle: Sequences of the two MOs used in the described studies (MO#1 = blue; MO#2 = red). They are perfectly complementary to the endogenous mRNA. Bottom: wbp2nl ORF is preceded by 6 in-frame copies of the myc epitope sequence. Thus, in the rescue mRNA, the wbp2nl ORF is precede by a long myc sequence (part of which in shown in lavender), the translational start site of which is 350 base pairs upstream of the binding site of MO#2. In addition, MO#1 is not complementary to this mRNA. Thus, the rescue mRNA is resistant to MO-mediated knock-down, as demonstrated in Supplemental Fig. 3.
Image published in: Marchak A et al. (2017)
Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-158004