Figure 1 β-Trcp forms a complex with β-catenin and Axin in vivo. (A and B) Association between β-Trcp and β-catenin. (A) Immunoprecipitation of β-Trcpmyc with an anti-myc antibody. Embryo extracts were from stage 9 embryos expressing β-catenin, β-catenin (S→A), or β-Trcpmyc alone or in combination. The precipitates were examined for β-catenin (Upper) and for β-Trcpmyc (Lower). A longer exposure revealed that β-Trcpmyc also coprecipitated endogenous β-catenin (data not shown). (B) Immunoprecipitation of β-catenin using the same embryo extracts as in A. The precipitates were examined for β-Trcpmyc (Upper) and for β-catenin (Lower). Endogenous β-catenin is seen in lanes 1 and 4. RNA injected per embryo: 1 ng each for β-catenin, β-catenin (S→A), and β-Trcpmyc. (C and D) A complex formation between β-Trcp and Axin mediated by β-catenin. (C) Immunoprecipitation of β-Trcpmyc. The extracts were from stage 9 embryos expressing β-Trcpmyc plus AxinFlag in the presence or absence of β-catenin or β-catenin (S→A). The precipitates were examined for β-catenin (Top), AxinFlag (Middle), and β-Trcpmyc (Bottom). (D) Immunoprecipitation of AxinFlag using the same extracts as in C. The precipitates were examined for β-Trcpmyc (Top), β-catenin (Middle), and AxinFlag (Bottom). RNA injected per embryo: same as in A and B except 2 ng for AxinFlag.
Image published in: Liu C et al. (1999)
Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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