Figure 2. Relative to guide RNA rhosg3, rhosg1 causes greater reductions in rod opsin and greater rod photoreceptor loss. 6 ng of Cas9 mRNA and 2 ng of sgRNA were co-injected. (A) Rod opsin levels assayed in individual eyes by dot blot assay at 14 dpf. Each data point represents a different animal. P values are for Tukey multiple comparisons test for comparisons with WT group. P (ANOVA) = 2.9 × 10−9 (B) Phenotypes assessed by confocal microscopy. Green: anti-rhodopsin (B630N). Red: wheat germ agglutinin. Blue: Hoechst 33342. RPE: retinal pigment epithelium. OS: outer segments. IS: inner segments. ONL: outer nuclear layer. INL: inner nuclear layer. Bar = 50 µm.
Image published in: Feehan JM et al. (2017)
© The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| rho.L | opn2, rh1, rhodopsin, rp4, xrho | X. laevis | Sometime during NF stage 48 to NF stage 49 | eye retina retinal neural layer photoreceptor layer eye photoreceptor cell |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||||||
|---|---|---|---|---|---|---|---|---|
| Xla Wt + rho CRISPR + NF49 (Other Detection Assay) | Fig.2.A |
| ||||||
| Xla Wt + rho CRISPR + NF49 (Other Detection Assay) | Fig.2.A |
| ||||||
| Xla Wt + rho CRISPR + NF49 (immunohistochemistry) | Fig.2.B |
| ||||||
| Xla Wt + rho CRISPR + NF49 (immunohistochemistry) | Fig2.B |
|
Permanent Image Page
Printer Friendly View
XB-IMG-158399