Figure 8: adam13 induction 926 of Ap2 and pcdh8l does not involve Smad2. (A-B) Relative expression of AP2 and pcdh8l in animal caps dissected from control embryos, or embryos injected with adam13 or adam13 and a morpholino to Smad2 (MOSmad2, 25 ng). C) Luciferase assays in Hek293T cells show no induction of AP2α promoter activity by Smad2 (0.5 μg) or Smad 7 (0.5 μg). Smad2 does not increase adam13 activation of the AP2 promoter, while Smad7 does not reduce adam13 induction of AP2 promoter. Smad2 and Smad7 were transfected with or without adam13 (0.5 μg) along with AP2α promoter-luciferase and the pRL-CMV (100 ng, 10 pg). The ratio of luciferase to renilla was used to normalize each transfection to the empty vector control (CS2). Error bars represent standard error to the mean (Mean± S.E.M). One-way ANOVA was performed to determine statistical significance. Statistically significant at **p < 0.01, ***p < 0.001.
Image published in: Khedgikar V et al. (2017)
© 2017, Khedgikar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
Permanent Image Page
Printer Friendly View
XB-IMG-158903