Figure 7. adam13 requires arid3a for induction of AP2α.Relative expression of AP2α (A) and pcdh8l (B) in animal caps from embryos injected with adam13 alone or with a morpholino to arid3a (20 ng) or FoxD3 (20 ng). Animal caps were extracted at stage 17.10.7554/eLife.26898.020Figure 7—source data 1. Source data for Figure 7.Figure 7—figure supplement 1. adam13 does not induce arid3a and FoxD3 expression in Naïve ectoderm.Animal caps were dissected from stage 9 embryos injected with the various constructs or morpholinos as indicated. RNA was extracted from AC at stage 20. Graphs show relative mRNA expression normalized to GAPDH for (A) arid3a and (B) FoxD3. Error bars represent standard error to the mean for three or more independent experiments. One-way ANOVA was performed to determine statistical significance. Statistically significant at *p<0.05, ***p<0.005.10.7554/eLife.26898.019Figure 7—figure supplement 1—source data 1. Source data for Figure 7—figure supplement 1.
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