Figure 1. Angiopoietin-like 4 Inhibits Wnt/b-Catenin Signaling at the Receptor Level (A) Wnt luciferase reporter assay in H1703 cells transfected with the indicated siRNAs, followed by treatment with Wnt3a or control (Co) conditioned medium. siAPC and sibcatenin were included as positive and negative control, respectively. (B) Wnt reporter assay in HEK293T cells transfected with siANGPTL4 or control, followed by treatment with the indicated conditioned media. (C) qRT-PCR analysis of AXIN2 expression in H1703 cells transfected with the indicated siRNAs. (D) Western blot analysis of cytosolic b-catenin in H1703 cells, following transfection with the indicated siRNAs and treatment with control (Co) or Wnt3a conditioned medium. (E) Wnt reporter epistasis assay in HEK293T cells. Cells were transfected with the indicated plasmids or siRNAs. (F) Western blot analysis of (activated) phospho-LRP6 in H1703 membrane fractions. Cells were treated with the indicated siRNAs, followed by treatment with Wnt3a or control (Co) conditioned medium. Data in all graphs are displayed as means ± SD, and show one representative of multiple independent experiments with three biological replicates. RLA, relative luciferase activity. *p < 0.05; **p < 0.01; ***p < 0.001. See also Figure S1.
Image published in: Kirsch N et al. (2017)
Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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