Figure 4 Examples of Pax-6 RNA injected embryos. In panels (A–C), anterior is to the right and dorsal is up. (A) An antisense injected embryo at stage 42 appears normal. It is representative of the mean DAI value for antisense RNA injection: DAI value = 5. (B) A sense injected embryo at control stage 42. The tail is shortened and bifurcated, but the anterior structures, including eye (arrow) and cement gland (arrowhead), appear to be normal. It is representative of the mean DAI value for sense RNA injection: DAI value = 7. (C) A sense injected embryo displaying a severe phenotype. Tail structures are absent and the neural tube has failed to close (arrow). The only recognizable anterior structure is the cement gland (arrowhead). DAI value = 10. (D) Summary of results from Pax-6 RNA injection experiments. S.E.M. = standard error of the mean. Scale bar = 200um
Image published in: Hirsch N and Harris WA (1997)
Copyright © 1997. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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