Supplementary Figure 8 Detailed localisation analysis of undifferentiated marker genes in il-11 KD tadpoles. a-d, WISH images for undifferentiated marker genes using cas9 mRNA-injected (a) or il-11 KD #1 (b-d) tadpoles that are not shown in Fig. 5. Representative WISH images for not (b), dcx (c), or runx1 (a and d) are shown. e-j, WISH images for undifferentiated marker genes using il-11 KD #2 tadpoles. Representative WISH images for not (e and h), dcx (f and i), or runx1 (g and j) are shown. k-q, Section images. Representative images for not (k), dcx (l, n and p), or runx1 (m, o and q) using cas9 mRNA-injected (k-m), il-11 KD #1 (n and o), or #2 (p and q) are shown. Anterior is to the left and dorsal is up. Blue/purple colour represents signals for each gene. Brown pigments are melanophores of the tadpoles. Scale bars: 500 μm (a-j) or 100 μm (k-q). Numbers at the bottom corner are the total ratio of tadpoles showing the corresponding expression from two batches. Green broken lines indicate spinal cord and spinal cord ampulla, and yellow broken lines indicate notochord and notochord bud.
Image published in: Tsujioka H et al. (2017)
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