Fig 5. Identification of gene ontology (GO) biological functions enriched in weighted gene co- expression network analysis (WGCNA) groups. (A) Distribution of Xenopus laevis transcripts in WGCNA groups. The color-filled bars on the histogram indicate the number of unique Entrez geneID records per group. Functions of some developmentally relevant groups are highlighted: the Wnt responding genes in blue (Groups #23 and #41 in blue), neural crest (#51, purple), forebrain (#38, orange), mRNA splicing (#80, pink), and cilium morphogenesis in green (#22, green). Refer to S6 and S6B Table for the full list of functions associated with each group. (B) Comparison between groups generated by WGCNA (blue) and those created by random assignment of transcripts (red). About 45% of the WGCNA groups associate to at least 1 biological function, which is significantly more than the average obtained for the random dataset groups (4%; the 5 random datasets of 141 groups). The distribution of adjusted p-values and sensitivities (1-β) of overrepresented terms show that, overall, WGCNA groups are more likely to contain a relevant biological function than groups assigned randomly. (C) Specificity versus sensitivity plot, in which each of the data points represents a single GO function enriched in a group. Black circles represent all 80 biological functions enriched in the 5 random datasets (total of 705 groups). The Wnt responding genes (Groups #23, blue), neural crest (#51, purple), forebrain (#38, orange) are highlighted. For further description of the function, refer to S6B Table; see S11 Table for numerical data.
Image published in: Plouhinec JL et al. (2017)
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