Fig. 2. BMP signaling coordinates D-V patterning. (A) Experimental design. FG and HG (endoderm plus mesoderm) explants were dissected from DMSO (vehicle)- or DMH1-treated NF20 embryos and submitted for RNA-seq (in triplicate). (B) Venn diagram showing overlap of FG-enriched genes, HG-enriched genes and BMP-activated and BMP-repressed transcripts (log2 FC≥1, FDR≤5%); see Fig. S2B for details. (C) Expression heatmap clustering of FG endoderm-, FG mesoderm-, HG endoderm- and HG mesoderm-enriched transcripts in vehicle-treated (CONT) or DMH1-treated explants. (D) Different categories of BMP-activated (ACT) or BMP-repressed (REP) transcripts based on whether the gene is normally enriched in the endoderm, mesoderm or expressed in both (en=me). (E) In situ hybridization of vehicle control-treated, DMH1-treated or BMP2-injected stage NF20 embryos in mid-sagittal section (cdx2, sfrp5, cebpa and nkx2-5; anterior left and dorsal up) or cross-section (hrg and mnx1; dorsal up; white lines indicate expression domain); n>20 for each probe.
Image published in: Stevens ML et al. (2017)
Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cdx2.L | cad2, cdx-2, cdx3-like, xcad2, Xcaudal2 | X. laevis | Throughout NF stage 20 | hindgut mesoderm endoderm circumblastoporal collar neuroectoderm |
| sfrp5.L | frp5, sFRP-5, sfrp5-a, sfrp5-b, XsFRP5 | X. laevis | Throughout NF stage 20 | foregut endoderm archenteron floor |
| cebpa.L | C/EBP, C/EBPalpha, xC/EBP | X. laevis | Throughout NF stage 20 | foregut mesoderm ventral mesoderm |
| nkx2-5.L | AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 | X. laevis | Throughout NF stage 20 | foregut mesoderm |
| fetub.S | fetuin-b, gugu | X. laevis | Throughout NF stage 20 | endoderm gastrocoel roof plate ectoderm |
| mnx1.L | hb9, hlxb9, hoxhb9, scra1 | X. laevis | Throughout NF stage 20 | endoderm gastrocoel roof plate neural tube |
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