Figure 8. Basolateral treatment with SMase diminishes transepithelial currents in HBEs: (a) An example trace showing that pretreatment with basolateral WT SMase diminishes FSK-stimulated transepithelial currents in HBEs expressing WT CFTR. Scale bars: 10 μA/cm2, 10 mins. (b) Summary data showing significant difference in current amplitudes following basolateral WT SMase (p < 0.0001 for FSK and SMase treatments, 2-way ANOVA; asterisks indicate p < 0.05 for subsequent pairwise t-test analyses). Inset-Fractional inhibition is significantly higher in the presence of 100 nM FSK than 100 μM FSK (p = 0.0016, t-test). (c) An example trace showing that pretreatment of temperature-corrected CFHBEs with basolateral SMase leads to a similar reduction in maximal current. Scale bars: 2 μA/cm2, 20 mins. (d) Summary data showing that basolateral treatment with WT SMase reduces currents elicited by 100 μM FSK + 1 μM VX-770 (NHBE p = 0.04, CFHBE-27 °C p = 0.02; t-test).
Image published in: Stauffer BB et al. (2017)
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