Figure 6. Mediolateral Cell Intercalation Behaviors in FN Knockdown Explants Are Rescued when Cells Provided with a Planar FN-Substrate while Blocking Integrin α5β1 Stimulates Cell Protrusive Activity (A and B) Two frames from representative confocal time-lapse sequences of 15 μMoles FNMO-injected explants expressing GAP43-GFP and their calculated “difference” images were calculated for explants cultured on BSA ([A]; see Movie S3) and on fibronectin ([B]; see Movie S4). A “difference time-lapse” sequence shows regions of cell protrusion and retraction as solid white and black regions, respectively, while regions where little change occurs are typified by gray. (C) Culture of FNMO-injected explants on FN significantly represses the rate of protrusions. (D–F) Two frames from a representative confocal time-lapse sequence and their calculated “difference” image before (D) and after ([E]; see Movies S5 and S6 for difference time-lapse sequence) addition of 0.1 μg/ml of the integrin α5β1 function-blocking antibody P8D4 to the explant show that protrusive behaviors are stimulated and the rate of protrusive activity increases (F). The time-lapse sequences, in two-level form, and the difference time-lapse sequence can be viewed in Supplemental Data.
Image published in: Davidson LA et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
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