Figure 5. The photoreceptor outer segments are misaligned in pcdh15 morphant larvae. 3D rendering of the confocal stacks obtained from acrylamide-embedded vibratome sections (150 µm thick) stained with fluorescent lectin (top) and from SEM micrographs (bottom), highlighting the orderly arrangement of the subretinal space in 4 dpf control retinas (left). In contrast, age-matched morphant retinas contain photoreceptors with misshapen, curved, and misaligned outer segments (OS; middle). The coinjection into the embryo of cRNAs encoding the protocadherin-15 CD1 and CD3 isoforms with the splice-blocking morpholinos essentially preserved the well-ordered organization and parallel alignment of the OS in the larva (right). IS, inner segment, C, cone, R, rod. Bars, 10 µm.
Image published in: Schietroma C et al. (2017)
© 2017 Schietroma et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license
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