Figure 2—figure supplement 2. Complementation of brush by overexpression of BRUSH.(A) Nodulation and rhizobial infection assay upon hairy root transformation of the brush mutant with an empty vector control or an UBQpro:BRUSHgenomic construct compared to the wild-type (Gifu) hairy roots transformed with empty vector. Infected nodules were observed in brush roots constitutively expressing the BRUSH genomic sequence. Shown are bright-field images of roots and DsRED fluorescence of rhizobia. (B) Rhizobia remain entrapped in infection pockets in brush root hairs (left image, arrow). Overexpression of BRUSH restores infection of root hairs (middle) leading to infected nodule cells (right). The root hair images are composites of bright field and DsRED fluorescence. Plants with hairy roots were grown in Weck jars, cultivated at 26°C, and inoculated with M. loti expressing DsRed. Roots were analyzed 4 weeks after inoculation. Scale bars represent 0.5 mm in the top three panels of (A), lower panel 2 mm. Scale bars in (B) represent 15 µm.
Image published in: Chiasson DM et al. (2017)
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