Figure 4. Rapgef5 Is Required for the Nuclear Localization of β-Catenin (A and B) GFP-tagged WT and ST β-catenin localize to the plasma membrane and nucleus in the dorsal blastopore lip of stage 10 control embryos but this nuclear localization is lost in rapgef5 morphants (note loss of nuclear GFP signal in merged images). (C) NLS β-catenin-GFP localizes normally into nuclei even in the absence of Rapgef5. Graphs in the center panel represent the ratio of nuclear-localized GFP relative to nuclear-localized NLS-cherry control. Controls were normalized to ease comparison. The graphs on the right display ratiometric analysis of nuclear versus cytoplasmic GFP (β-catenin) and NLS-mCherry levels. Nuclear-localized β-catenin is reduced in Rapgef5 morphants relative to controls for WT and ST β-catenin, but not NLS-β-catenin. Localization of NLS-mCherry is unaltered. All data represented as mean ± SE. ∗∗p < 0.01, ∗∗∗p < 0.005.
Image published in: Griffin JN et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| ctnnb1 | B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin | X. tropicalis | Sometime during NF stage 10 to NF stage 42 | dorsal marginal zone upper blastopore lip |
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