Larger Image Figure 4: In situ hybridization of ism1 expression in late stage Xenopus laevis embryos. Embryos are shown in a lateral (A-D) or anterior (A’-D’) view with the cement gland (cg) labeled for ventral orientation. (A) Stage 28 embryo showing strong expression in the branchial arches (ba), midbrain-hindbrain boundary (mhb), ear placode (e), and tailbud (tb) with the yellow arrowheads marking the primitive mouth. (B) Stage 33 embryos showing decreased expression in the branchial arches (ba), midbrain-hindbrain boundary (mhb) and tailbud (tb), with concentrated expression surrounding the primitive mouth (arrowhead) and expression in the somites (s). (C-D) Stage 37 and 39 embryos showing continued concentrated ism1 expression surrounding the primitive mouth (arrowhead). Image published in: Lansdon LA et al. (2018) Copyright © 2018. This image is reproduced with permission of the journal and the copyright holder. This permission was brokered through the Copyright Clearance Centre (CCC).
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