Figure 2. MarvelD3 depletion disrupts neural crest derivatives development. (A) Craniofacial cartilage morphology was analysed and its size measured in control, MD3AB morpholino-injected embryos and embryos co-injected with 7mut-marveld3 mRNA. Note, ceratohyal (red arrowhead) and ceratobranchial (white arrow) cartilage was reduced in MarvelD3 morphants. (B) The lateral line pigmentation (stage 42) pattern was analysed and quantified in control morpholino, MD3AB morpholino-injected embryos and embryos co-injected with 7mut-marveld3 mRNA. Scale bars, 500 μm; Student t-test p values and the number of embryos analysed are indicated on the graph; blue bar, normal pigments; red bar, reduced pigments; the number craniofacial cartilage samples analysed is indicated on the graph; black bar, wt; red bar, MD3AB morpholinos; light red bar, MD3AB morpholinos + 7mut-marveld3 RNA. (C) 7mut-marveld3 was subcloned in pCS2+ vector (BamH1/ EcoR1). Restriction sites: underlined; mutated bases of marveld3: underlined lower cases.
Image published in: Vacca B et al. (2018)
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