Figure 5. MAPK and PI3K/Akt differentially alter the transit of pluripotent cells to restricted cell states.(A–B) Animal pole explant assay examining expression of Xbra and MyoD (A) or Endodermin and Sox17 (B) in explants cultured with or without activin after treatment with Meki (RDEA119) or injection with dnPI3K. Explants were cultured alongside sibling embryos and collected at midgastrula stages (stage 11.5) for Xbra, Endodermin, and Sox17 expression and midneurula stages (stage 15/16) for MyoD expression. Blocking either cascade interferes with mesoderm formation whereas only MAPK signaling is required for Endodermin induction.Figure 5—figure supplement 1. MAPK and PI3K/Akt signaling are differentially required for Endodermin expression.(A) Animal pole explant assay examining expression of Endodermin in explants cultured with or without activin after injection with dnRaf or treatment with PI3Ki (L) (LY294) or PI3Ki (W) (Wortmannin). Explants were cultured alongside sibling embryos and collected at midgastrula stages (stage 11.5). MAPK signaling is required for Endodermin induction.
Image published in: Geary L and LaBonne C (2018)
© 2018, Geary et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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