Figure S3. Visual Preference Assay. Surgery was performed on st. 27 embryos to remove left eye tissues. To assay for visual preference only for the regrown eye, the right eye was removed at st. 40 (no regrowth is seen for embryo eye at this stage (Blackiston and Levin, 2013; Sedohara et al., 2003)). Embryos were raised under a 12 hour light:dark schedule. Visual preference for the white versus black colors were performed based on protocol described in (Viczian and Zuber, 2014) and modified as follows: individual swimming tadpoles were transferred to a 15x20 cm container with half white and half black background made using black and white tape. A single tadpole was placed in the black side of the container. Each tadpole was given about eight minutes to acclimate to the new environment. For the assay, the time that tadpoles spent in each colored background was measured within a two-minute timeframe. The same set of tadpoles were retested the following day. The results were the same for both days. For each condition, at least 10 tadpoles from each of three different batches of embryos were assayed. Tadpoles with one regrown eye display similar white background preference as control tadpoles with one normal eye (n=10 per condition, p>0.5). Blind tadpoles do not show preference for the white background (n=10, * denotes p<0.01 when compared to other conditions).
Image published in: Kha CX et al. (2018)
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