Figure 7. xNuRD Is Required for Development and DNA Replication before the MBT In Vivo(A and B) Phenotypes of developing Xenopus embryos after microinjection of NuRD-specific antibodies and Y3 RNA-specific antisense morpholino oligonucleotides (MOs). The indicated antibodies (panel A, 65ng serum/embryo; panel B, 5ng IgG/embryo) and MOs (control coMO or Y3 MO, 40 ng/embryo) were injected into the animal pole at the 1-cell stage, and representative embryos were photographed at the indicated times post fertilization (p.f.). See Movies S1 and S2 for the full time course of these experiments.(C and D) DNA replication before the MBT depends on xNuRD. Total embryonic DNA of experimental repeats shown in (A) and (B) was quantified relative to rRNA (C) and (D), respectively. Individual datasets were normalized to the mean of the control distributions and plotted as box and whisker plots superimposed with individual data points. Antibodies available to other xNuRD subunits were ineffective in this assay, regardless of whether Y RNA was co-depleted (Figure S6). Results of t tests (unpaired, two-tailed with unequal variance) of treated experimental samples against the controls are indicated (∗p = 0.07; ∗∗∗p ≤ 0.001).See also Figures S5 and S6 and Movies S1 and S2.
Image published in: Christov CP et al. (2018)
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