Fig. 8. Effects of chemicals on signal activation of TGF-β, ERK and ROS. Tail-amputated wild-type tadpoles were treated with DMSO (Control), or inhibitors for ERK (ERK i, U0126), ROS (ROS i, DPI) or TGF-β (TGFβ i, SB431542) signal. Upper and lower panels show fluorescence and normal light images in the same fields. (A, B) Representative images stained with anti-phospho Smad2 at 6 hpa (A) and anti-phospho ERK at 6 hpa (B). “–Ab” indicates control without 1st antibody. Arrowheads indicate specific signals in the stump epidermis. Arrows indicate non-specific fluorescence in deep region. (C) Representative images stained with H2DCFDA at 9 hpa. “–Dye” indicates control without incubation in H2DCFDA. Arrowheads indicate sites of ROS signaling activation. Anterior left, dorsal up. Bars, 100 µm.
Image published in: Sato K et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| smad2.L | smad-2, XSmad2 | X. laevis | Sometime during NF stage 49 to NF stage 50 | regenerating tail |
| mapk1.L | erk, erk1, erk2, ert1, MAP kinase, mapk, mapk1-a, mapk1-b, mapk2, mpk1, p42mapk, prkm1, prkm2, xp42 | X. laevis | Sometime during NF stage 49 to NF stage 50 | regenerating tail |
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