Figure 1. Hypoxia affects proliferation of progenitors in the retinal stem cell niche in the opposite manner, dependent on the nutritional status. (A) Schematic representation of a cross section through a 38-stage Xenopus retina, indicating the position of the ciliary marginal zone (CMZ). (B–E) EdU incorporation measured after a 2 h EdU pulse in DAPI-stained retinas from animals that have been normal-fed (B,C) or nutrient-deprived for 24 h (D,E) incubated under normoxia (B,D) or hypoxia (C,E). Nutrient deprivation or hypoxia reduced the number of proliferating cells in the CMZ, whereas hypoxic nutrient-deprived retinas show an increase of proliferation in the CMZ. Scale bars = 50 µm. For each condition, between 10 and 15 retinas were quantified. (F) Quantification of the experiment performed in (B–E). Error bars represent standard deviations. Two hooked ends of horizontal lines indicate the two conditions compared, *** p-value < 0.001; n = 7. (G) Quantification of EdU-positive cells after an 1 h EdU incorporation in retinas from normal-fed (blue line) or nutrient-deprived (red line) animals after various times in hypoxia (time in minutes indicated on the x-axis). Error bars represent standard deviations of two independent experiments (n = 2). For each condition and time point, a minimum of 10 retinas was quantified.
Image published in: Khaliullina H et al. (2016)
© 2016 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + hypoxia + NF38 |
| |||
| Xla Wt + nutrient deprivation + NF38 |
| |||
| Xla Wt + nutrient deprivation + hypoxia + NF38 |
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